Vertical nanowire arrays as a versatile platform for protein detection and analysis.
Identifieur interne : 000282 ( Main/Exploration ); précédent : 000281; suivant : 000283Vertical nanowire arrays as a versatile platform for protein detection and analysis.
Auteurs : RBID : pubmed:24062006English descriptors
- KwdEn :
- Animals, Arsenicals (chemistry), Biotin (chemistry), Biotin (metabolism), Cattle, Fluorescent Dyes (chemistry), Immobilized Proteins (chemistry), Immobilized Proteins (metabolism), Immunoassay, Indium (chemistry), Maleimides (chemistry), Nanowires (chemistry), Nickel (chemistry), Nitrilotriacetic Acid (chemistry), Protein Array Analysis (instrumentation), Proteins (analysis), Serum Albumin (chemistry), Streptavidin (chemistry), Streptavidin (metabolism).
- MESH :
- chemical , analysis : Proteins.
- chemical , chemistry : Arsenicals, Biotin, Fluorescent Dyes, Immobilized Proteins, Indium, Maleimides, Nickel, Nitrilotriacetic Acid, Serum Albumin, Streptavidin.
- chemical , metabolism : Biotin, Immobilized Proteins, Streptavidin.
- chemistry : Nanowires.
- instrumentation : Protein Array Analysis.
- Animals, Cattle, Immunoassay.
Abstract
Protein microarrays are valuable tools for protein assays. Reducing spot sizes from micro- to nano-scale facilitates miniaturization of platforms and consequently decreased material consumption, but faces inherent challenges in the reduction of fluorescent signals and compatibility with complex solutions. Here we show that vertical arrays of nanowires (NWs) can overcome several bottlenecks of using nanoarrays for extraction and analysis of proteins. The high aspect ratio of the NWs results in a large surface area available for protein immobilization and renders passivation of the surface between the NWs unnecessary. Fluorescence detection of proteins allows quantitative measurements and spatial resolution, enabling us to track individual NWs through several analytical steps, thereby allowing multiplexed detection of different proteins immobilized on different regions of the NW array. We use NW arrays for on-chip extraction, detection and functional analysis of proteins on a nano-scale platform that holds great promise for performing protein analysis on minute amounts of material. The demonstration made here on highly ordered arrays of indium arsenide (InAs) NWs is generic and can be extended to many high aspect ratio nanostructures.
DOI: 10.1039/c3nr03113f
PubMed: 24062006
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Vertical nanowire arrays as a versatile platform for protein detection and analysis.</title><author><name sortKey="Rostgaard, Katrine R" uniqKey="Rostgaard K">Katrine R Rostgaard</name><affiliation wicri:level="1"><nlm:affiliation>Bio-Nanotechnology and Nanomedicine Laboratory, Department of Chemistry & Nano-Science Center, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark. martinez@nano.ku.dk.</nlm:affiliation><country xml:lang="fr">Danemark</country><wicri:regionArea>Bio-Nanotechnology and Nanomedicine Laboratory, Department of Chemistry & Nano-Science Center, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen</wicri:regionArea></affiliation></author><author><name sortKey="Frederiksen, Rune S" uniqKey="Frederiksen R">Rune S Frederiksen</name></author><author><name sortKey="Liu, Yi Chi C" uniqKey="Liu Y">Yi-Chi C Liu</name></author><author><name sortKey="Berthing, Trine" uniqKey="Berthing T">Trine Berthing</name></author><author><name sortKey="Madsen, Morten H" uniqKey="Madsen M">Morten H Madsen</name></author><author><name sortKey="Holm, Johannes" uniqKey="Holm J">Johannes Holm</name></author><author><name sortKey="Nyg Rd, Jesper" uniqKey="Nyg Rd J">Jesper Nygård</name></author><author><name sortKey="Martinez, Karen L" uniqKey="Martinez K">Karen L Martinez</name></author></titleStmt><publicationStmt><date when="2013">2013</date><idno type="doi">10.1039/c3nr03113f</idno><idno type="RBID">pubmed:24062006</idno><idno type="pmid">24062006</idno><idno type="wicri:Area/Main/Corpus">000395</idno><idno type="wicri:Area/Main/Curation">000395</idno><idno type="wicri:Area/Main/Exploration">000282</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Arsenicals (chemistry)</term><term>Biotin (chemistry)</term><term>Biotin (metabolism)</term><term>Cattle</term><term>Fluorescent Dyes (chemistry)</term><term>Immobilized Proteins (chemistry)</term><term>Immobilized Proteins (metabolism)</term><term>Immunoassay</term><term>Indium (chemistry)</term><term>Maleimides (chemistry)</term><term>Nanowires (chemistry)</term><term>Nickel (chemistry)</term><term>Nitrilotriacetic Acid (chemistry)</term><term>Protein Array Analysis (instrumentation)</term><term>Proteins (analysis)</term><term>Serum Albumin (chemistry)</term><term>Streptavidin (chemistry)</term><term>Streptavidin (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Arsenicals</term><term>Biotin</term><term>Fluorescent Dyes</term><term>Immobilized Proteins</term><term>Indium</term><term>Maleimides</term><term>Nickel</term><term>Nitrilotriacetic Acid</term><term>Serum Albumin</term><term>Streptavidin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Biotin</term><term>Immobilized Proteins</term><term>Streptavidin</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanowires</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Protein Array Analysis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Immunoassay</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Protein microarrays are valuable tools for protein assays. Reducing spot sizes from micro- to nano-scale facilitates miniaturization of platforms and consequently decreased material consumption, but faces inherent challenges in the reduction of fluorescent signals and compatibility with complex solutions. Here we show that vertical arrays of nanowires (NWs) can overcome several bottlenecks of using nanoarrays for extraction and analysis of proteins. The high aspect ratio of the NWs results in a large surface area available for protein immobilization and renders passivation of the surface between the NWs unnecessary. Fluorescence detection of proteins allows quantitative measurements and spatial resolution, enabling us to track individual NWs through several analytical steps, thereby allowing multiplexed detection of different proteins immobilized on different regions of the NW array. We use NW arrays for on-chip extraction, detection and functional analysis of proteins on a nano-scale platform that holds great promise for performing protein analysis on minute amounts of material. The demonstration made here on highly ordered arrays of indium arsenide (InAs) NWs is generic and can be extended to many high aspect ratio nanostructures.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">24062006</PMID><DateCreated><Year>2013</Year><Month>10</Month><Day>11</Day></DateCreated><DateCompleted><Year>2014</Year><Month>05</Month><Day>14</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2040-3372</ISSN><JournalIssue CitedMedium="Internet"><Volume>5</Volume><Issue>21</Issue><PubDate><Year>2013</Year><Month>Nov</Month><Day>7</Day></PubDate></JournalIssue><Title>Nanoscale</Title><ISOAbbreviation>Nanoscale</ISOAbbreviation></Journal><ArticleTitle>Vertical nanowire arrays as a versatile platform for protein detection and analysis.</ArticleTitle><Pagination><MedlinePgn>10226-35</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c3nr03113f</ELocationID><Abstract><AbstractText>Protein microarrays are valuable tools for protein assays. Reducing spot sizes from micro- to nano-scale facilitates miniaturization of platforms and consequently decreased material consumption, but faces inherent challenges in the reduction of fluorescent signals and compatibility with complex solutions. Here we show that vertical arrays of nanowires (NWs) can overcome several bottlenecks of using nanoarrays for extraction and analysis of proteins. The high aspect ratio of the NWs results in a large surface area available for protein immobilization and renders passivation of the surface between the NWs unnecessary. Fluorescence detection of proteins allows quantitative measurements and spatial resolution, enabling us to track individual NWs through several analytical steps, thereby allowing multiplexed detection of different proteins immobilized on different regions of the NW array. We use NW arrays for on-chip extraction, detection and functional analysis of proteins on a nano-scale platform that holds great promise for performing protein analysis on minute amounts of material. The demonstration made here on highly ordered arrays of indium arsenide (InAs) NWs is generic and can be extended to many high aspect ratio nanostructures.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rostgaard</LastName><ForeName>Katrine R</ForeName><Initials>KR</Initials><Affiliation>Bio-Nanotechnology and Nanomedicine Laboratory, Department of Chemistry & Nano-Science Center, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark. martinez@nano.ku.dk.</Affiliation></Author><Author ValidYN="Y"><LastName>Frederiksen</LastName><ForeName>Rune S</ForeName><Initials>RS</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yi-Chi C</ForeName><Initials>YC</Initials></Author><Author ValidYN="Y"><LastName>Berthing</LastName><ForeName>Trine</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Madsen</LastName><ForeName>Morten H</ForeName><Initials>MH</Initials></Author><Author ValidYN="Y"><LastName>Holm</LastName><ForeName>Johannes</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Nygård</LastName><ForeName>Jesper</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Martinez</LastName><ForeName>Karen L</ForeName><Initials>KL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>09</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nanoscale</MedlineTA><NlmUniqueID>101525249</NlmUniqueID><ISSNLinking>2040-3364</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Alexa Fluor 488 C5-maleimide</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Arsenicals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Fluorescent Dyes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Immobilized Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Maleimides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Serum Albumin</NameOfSubstance></Chemical><Chemical><RegistryNumber>045A6V3VFX</RegistryNumber><NameOfSubstance>Indium</NameOfSubstance></Chemical><Chemical><RegistryNumber>1303-11-3</RegistryNumber><NameOfSubstance>indium arsenide</NameOfSubstance></Chemical><Chemical><RegistryNumber>6SO6U10H04</RegistryNumber><NameOfSubstance>Biotin</NameOfSubstance></Chemical><Chemical><RegistryNumber>7OV03QG267</RegistryNumber><NameOfSubstance>Nickel</NameOfSubstance></Chemical><Chemical><RegistryNumber>9013-20-1</RegistryNumber><NameOfSubstance>Streptavidin</NameOfSubstance></Chemical><Chemical><RegistryNumber>KA90006V9D</RegistryNumber><NameOfSubstance>Nitrilotriacetic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Arsenicals</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Biotin</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cattle</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Fluorescent Dyes</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Immobilized Proteins</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Immunoassay</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Maleimides</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nanowires</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nickel</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nitrilotriacetic Acid</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Protein Array Analysis</DescriptorName><QualifierName MajorTopicYN="Y">instrumentation</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Proteins</DescriptorName><QualifierName MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Serum Albumin</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Streptavidin</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate 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